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The captured troponin is then reacted by using a next antibody and, in certain assays, a 3rd antibody coupled to an indicator molecule.[36] The assays differ from one another by the types of antibody utilized, because of the epitopes to which they bind, and by the kind of indicator molecule that may be utilized.[forty six]
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Two troubles limit the benefit of switching from one particular troponin assay to a different in clinical observe or analysis. Very first, no Main reference cTnI product is currently available for manufacturers to standardize cTnI assays.
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Final results could possibly be confounded when there is lousy preanalytical managing of troponin specimens, which include incomplete sample mixing at the time of specimen collection, insufficient sample centrifugation and separation of red cells from serum or plasma, presence of fibrin as a result of incomplete serum separation, and so forth.